Sample-Based Incidence (ESTIMATES- TUTORIAL)

1) Take your morphospecies information (Figure 1).  For example (farms are not shown in this figure), we have the morphospecies listed here. So we are going to do the sample-based analysis based on different shade levels (Low vs. High). We are going to see how the diversity of parasitoid wasps (using diversity estimators) differ between high-shade farms and low-shade farms.

                                                                       (Figure 1) 

2) Use the sort button in Excel to divide the farms like Figure 2. Overall, we have 38 sites but split into 19 low-shade farms and 19 high-shade farms.

(Figure 2)

3) Open a new excel worksheet because I think it is easier! Though you can just take the original file and just restructure it. Copy and paste (exclude the farmsites/shade column so just species information are present) like Figure 3.

(Figure 3) 

4) Now here is the most important part. You must be accurate with your titles. If you don't then EstimateS will spit out an error.

FIRST ROW: 

*MultipleSampleSets* 

(The number of datasets you are going to look at- 2 for this example because"high" or "low")

*Title of the overall file* [OPTIONAL]

(Figure 4) 

SECOND ROW: 

(Title of this particular dataset)

*SampleSet*

(The Format Code) 

(How many rows to skip)

(How many columns to skip)

(Figure 5)

Now with the format code, if you're looking at individuals per site use 2 (look at EstimateS userguide for more information)

THIRD ROW:

*The number of species all together*

*The number of sites in this particular dataset*

(Figure 6)

REPEAT.

(Figure 7)

5) Load the data into EstimateS

(Figure 8)